Ambrose Austin Lane was a farmer in the Potchefstroom district, North-West, from 1911 or earlier until his death. He was married three times: First, in Ireland, to Leonore Susan Arthurine Manning. They had no children. Second, still in Ireland, to Nelly Horneck, with whom he had two daughters. After her death he married Amy Lilian Blackman in England. They had a son, and she survived him.
In the Potchefstroom district Lane resided on the farm Mooibank, on the southern edge of the town, in 1919. By 1924 he had moved to the farm Machavie, and by 1932 to the farm Rietfontein.
Lane was a keen observer of birds and published several papers based on his observations: "Remarks on some widely distributed groups of birds containing distinctive family traits" (Journal of the South African Ornithologists' Union, 1915, Vol. 11(1), pp. 25-29); "Notes on the Charadridae or Plover family" (South African Journal of Natural History, 1920, Vol. 2(1), pp. 76-79); "Some notes on weavers and widow-birds" (The Ostrich, 1930, Vol. 1(1), pp. 22-24); and "Birds on Rietfontein farm, Loop Spruit Valley, Potchefstroom district" (The Ostrich, 1932, Vol. 3(1), pp. 17-20, with additional notes in 1933, Vol. 4(2), pp.71-74 and 1936, Vol. 7, pp. 39-44). In 1916 he became a foundation member of the South African Biological Society.